Device

Part:BBa_K759001:Design

Designed by: Ichiro Takeuchi   Group: iGEM12_HokkaidoU_Japan   (2012-09-19)


Aggregation Module inducible by arabinose in E.coli.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1945
    Illegal NgoMIV site found at 3169
    Illegal NgoMIV site found at 3679
    Illegal NgoMIV site found at 3700
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2695
    Illegal AgeI site found at 3439
    Illegal AgeI site found at 3853
    Illegal AgeI site found at 4095
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We found at that part (BBa_K542009) is lacking Lac1 promoter and RBS.So we need to construct it with basic parts. And add terminators.



Source

Peking 2010 team parts.

References